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The Polymerase chain reaction (PCR) is a technique used to amplify a single piece of DNA or RNA sequence exponentially to a magnitude of thousands of copies with the use of thermal cycling, primers and DNA polymerase. The polymerase enzymatically assembles a new sequence from free nucleotides and oligonucleotides (primers), by using the single-stranded DNA as a template, during the various stages of heating and cooling of the thermal cycle.