Open/Close Menu

TaKaRa Taq DNA Polymerase and Premix Taq DNA Polymerase

  • Description
  • Specifications
  • Downloads
  • Get Quote
A versatile and thermostable polymerase

TaKaRa Taq DNA Polymerase is a versatile and thermostable polymerase suitable for a variety of standard PCR applications. The enzyme is also supplied as a Premix Taq DNA polymerase (Cat. #R004A), a 2X PCR master mix containing Taq enzyme, buffer and dNTPs. This premix formulation simplifies PCR setup and minimizes pipetting steps. This is particularly helpful for high-throughput screening projects.

TaKaRa Taq DNA Polymerase is a recombinant and thermostable 94 kDa Taq polymerase encoded by a DNA polymerase gene derived from the Thermus aquaticus YT-1 strain and cloned intoEscherichia coli.TaKaRa Taq DNA Polymerase has been demonstrated to have the same characteristics and capabilities as native Taq DNA polymerase.


 

Features

  • Excellent for standard PCR amplifications
  • Low bacterial DNA contamination enzyme
  • Premix Taq enables easy PCR setup and minimizes pipetting steps

Applications

  • Routine PCR
  • DNA sequencing

Quality control results for TaKaRa Taq DNA Polymerase and a Taq enzyme from Company A. Because Taq DNA polymerase is typically expressed in E. coli, it is especially important to test the polymerase for the presence of contaminating E. coli genomic DNA. Quality control testing of TaKaRa Taq polymerase for contamination was performed by nested PCR of the E. coli genomic DNA Ori region. The results demonstrate that TaKaRa Taq enzyme is a low DNA contamination grade enzyme.

Quality control results for TaKaRa Taq DNA Polymerase and a Taq enzyme from Company A. Because Taq DNA polymerase is typically expressed in E. coli, it is especially important to test the polymerase for the presence of contaminating E. coli genomic DNA. Quality control testing of TaKaRa Taq polymerase for contamination was performed by nested PCR of the E. coli genomic DNA Ori region. The results demonstrate that TaKaRa Taq enzyme is a low DNA contamination grade enzyme.

Characterization:

Components

R001A

Takara Taq 250 U (5 U/µL)*
10X PCR Buffer (contains 15 mM MgCl2) 1 mL
dNTP Mixture (2.5 mM each dNTP) 800 µL

R001AM

Takara Taq 250 U (5 U/µL)*
10X PCR Buffer (without Mg2+) 1 mL
25 mM MgCl2 1 mL
dNTP Mixture (2.5 mM each dNTP) 800 µL

R004A – Premix Taq (Takara Taq Version)

2X Taq Polymerase Premix 6 x 500 µL**

*Protocol recommends the use of 1.25 U per 50 µL reaction.
**Contains Takara Taq, PCR Buffer and dNTPs.


 

Source

Recombinant E. coli


 

Storage

–20°C. Avoid repeated freeze-thaw of Takara Taq and dNTPs. Once thawed, aliquot into separate tubes and store at –20°C.


 

PCR Performance

Enzymatic performance is confirmed by successful amplification of an 8 kb lambda DNA template. PCR amplification of a single copy gene is also confirmed using a 2.9 kb human genomic DNA template and human p53 primers.


Technical Data:


You have nothing in your basket. Please click on the ‘Add To Cart’ button to request a quotation!