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SpectraMax 190

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 The multi-channel design truly mimics a dual-beam spectrophotometer. Each sample has a discrete sample beam and reference beam so that each well is measured directly, eliminating error due to variations in light output between the optic fibers.

See Comparison of Microplate Readers with Absorbance Detection

Performs Virtually any UV/VIS assay
> Cell biology
> Biochemistry
> Molecular biology
> Pharmacology
> Enzyme kinetics
> Environmental testing
> Entomology
> Bacterial identification
> Colorimetric
> Food/agricultural




UV-visible absorbance microplate reader for up to 96 samples with endpoint, kinetic and spectral scanning modes
190_microplate Features

    • Ability to monitor temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
    • Correction for varying microplate well volumes and the elimination of standard curves based on PathCheck® Technology, the only temperature-independent pathlength correction method
    • Thorough mixing of samples in microplate wells by user-programmable Automix feature
    • Seamless automation coupling with the StakMax Stacker, Molecular Devices’ own integrated microplate stacker designed exclusively for the SpectraMax Reader

Technical Data:


The patented multichannel optical design of the SpectraMax 190 Absorbance Microplate Reader mimics a dual beam spectrophotometer, measures each sample in the plate directly, and eliminates measurement error due to variations in light output between optical fibers on other instruments for more reliable data reporting.

Flexible Template Assignment

Flexible Template Assignment
Standards for multiple calibration curves and unknown can be run on separate plates

Technical Specifications

Temperature range: Ambient +4– 45°C
Temperature uniformity (microplate):
±0.5°C at 37°C, well-to-well
Temperature stabilization time:
30 minutes maximum upon initiation
Reading chambers: Isothermal when
temperature regulation
not enabled, < 1°C
General Specifications
Dimensions (in.): 8.6 (H) x 22.8 (W) x 15 (D)
Dimensions (cm): 22 (H) x 58 (W) x 38 (D)
Weight: 30 lbs. (13.6 kg)
Power requirements: 90–250 Vac 50/60 Hz
Power consumption: < 250 W
Operating temperature: 10–40°C
Storage temperature: 5–40°C
Accurate Measurements
The SpectraMax 190 Reader is the tool for DNA
analysis. The ability to detect 16 ng/well of DNA
and to quantitate 50 ng of DNA provides the
same sensitivity as a spectrophotometer. The
PathCheck Sensor can extend the instrument
linearity by a factor of three or more. For very
concentrated samples, use a minimum volume
in the microplate well (100 µL has a pathlength
of about 0.30 cm), then use the PathCheck
Sensor to calculate an equivalent 1-cm pathlength
The SpectraMax 190 Reader can be integrated
with Molecular Devices’ StakMax® Microplate
Stacker in a matter of minutes and begin reading
microplates with seven mouse clicks. For a
higher degree of automation, the Automation
Vendor Partners Program has streamlined the
integration of our microplate reader systems with
all leading partner robots. The “out-of-the-box”
automation solution saves up-front integration
time and resources.


Multiple calibration curves. Multiple calibration curves can be plotted on one graph


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