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Protino® Ni-NTA Columns

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Protino® Ni-NTA products enable fast and convenient purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). Proteins from any expression system can be purified under native or denaturing conditions. Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions.
Protino® Ni-NTA Agarose consists of the chelating ligand nitrilotriacetic acid (NTA) immobilized on 6 % cross-linked agarose beads that are suitable for batch binding, gravity-flow, and FPLC™ columns. The resin is precharged with Ni2+ ions and therefore ready to use.


Ready-to-use resin / columns for any application
Protino® Ni-NTA Agarose – bulk resin for batch purification, gravity flow columns and FPLC™
Protino® Ni-NTA Columns – prepacked ready-to-use FPLC™ columns

Characterization:

Protino® Ni-NTA products enable fast and convenient purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). Proteins from any expression system can be purified under native or denaturing conditions. Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions. 

Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification
• Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies)
• High capacity and high affinity
• Simply replace your current Ni-NTA products, no optimization or protocol change necessary
• Purification under native and denaturing conditions

 

Structure of NTA in complex with Ni2+


Technical Data:

FPLC™ purification of His-tagged green fluorescent protein and high-molecular, oligomeric His-tagged aspartase
Equivalent amounts of clarified E. coli lysate containing His-tagged green fluorescent protein (GFP) or aspartase were used as samlpe for the comparison (10 mL sample volume). Elution fractions were analyzed by SDS-PAGE.(A) For low molecular GFP monomer the cross-linked agarose from Protino® Ni-NTA Columns shows similar performance as competitor Q´s Ni-NTA Superflow Cartridges (26.7 mg compared to 21.7 mg). (B) For large proteins, such as high-molecular, oligomeric aspartase cross-linked agarose from Protino® Ni-NTA Columns show higher yield compared to the Ni-NTA Superflow Cartridges (52 mg compared to 24 mg).
Protino® Ni-NTA Columns 1 mL Protino® Ni-NTA Columns 5 mL
Technology IMAC (immobilized metal ion affinity chromatography) IMAC (immobilized metal ion affinity chromatography)
Chelating ligand NTA (nitrilotriacetic acid) NTA (nitrilotriacetic acid)
Format 1 mL FPLC™ columns 5 mL FPLC™ columns
Column dimensions 0.7 cm (inner diameter) x 2.5 cm (height) 1.6 cm (inner diameter) x 2.5 cm (height)
Column body material Polypropylen Polypropylen
Column inlet port 10-32 (1/16”) female 10-32 (1/16”) female
Column outlet port 10-32 (1/16”) male 10-32 (1/16”) male
Matrix 6 % beaded agarose (cross-linked), precharged with Ni2+ 6 % beaded agarose (cross-linked), precharged with Ni2+
Bead size 45–165 µm 45–165 µm

Binding capacity*

 50 mg 250 mg
Recommended flow rate**  1 mL/min 5 mL/min
Max. recommended flow rate 4 mL/min 10 mL/min
Storage solution 30 vol % ethanol 30 vol % ethanol
Storage temperature 4–8 °C 4–8 °C
* Binding capacity will vary for each polyhistidine-tagged protein
*
High flow rates may reduce binding capacity


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