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Protino® Ni-NTA Agarose

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Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification


Purification of His-tag proteins

Characterization:

Protino® Ni-NTA, the cost-effective NTA products for His-tag protein purification

• Chelating group NTA (nitrilotriacetic acid), two protein binding sites (also see Technologies)
• High capacity and high affinity
• Simply replace your current Ni-NTA products, no optimization or protocol change necessary
• Purification under native and denaturing conditions
• Suitable for small proteins, large protein complexes, proteins with low expression rates

Technology

IMAC (immobilized metal ion affinity chromatography)

Chelating ligand NTA (nitrilotriacetic acid)
Format

50 % (v/v) aqueous suspension containing 30 % ethanol

Matrix

6 % beaded agarose (cross-linked), precharged with Ni2+

Bead size

45–165 µm

Binding capacity* 50 mg/mL
Storage temperature

4–8 °C

* Binding capacity will vary for each polyhistidine-tagged protein


Technical Data:

Product

Qty

Specification
Protino® Ni-NTA Agarose

25 mL

settled beaded agarose

100 mL

as above

500 mL

as above
Product accessories

Qty

Specification
Protino® Columns 14 mL

10

empty columns (gravity flow)
for use with, e.g., Protino® Ni-NTA Agarose
Protino® Columns 35 mL

10

empty columns (gravity flow)
for use with, e.g., Protino® Ni-NTA Agarose


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