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PCR Setup STARlet

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The Hamilton PCR Setup Workstation offers the combination of a manually created PCR and automation;  this enables users to preserve the intuitiveness of manual setup, but to also benefit from not needing to do all the cumbersome “background work”.

 

The software consists of a graphical user interface (GUI) that easily guides through the setup process. Preconfigured algorithms and refined liquid handling parameters enable accurate setup of even low volume PCR assays in 384 plate format.

 

 

 

This standard solution provides user-friendly software and the option of several platform sizes – from the benchtop NIMBUS instrument to the STARline range for more demanding processes.

The PCR setup Workstation combines both the sample normalization and the PCR setup step in one system.

Up- and downstream processes such as sample quantification or PCR on- or off-deck can be implemented as optional add-ons.

pcr-setup-worflow


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The Hamilton PCR Setup Workflow

 

Characterization:

Features
  • Reaction setup for a wide range of PCR types in 96 and 384 PCR plate format but also strips or even rotor discs
  • Quick PCR setup, from simple two step reactions (templates + ready to use Master Mixes) up to 4 steps with separate primers, enzyme mixes and optional dilution step
  • Advanced PCR setup with up to 7 PCR components/steps and completely free PCR plate design
  • Normalization of input samples within a range of 1/10 000 to required target concentration
  • Multiplexing, by pipetting up to four primer mixes/ can also be used to pipette different PCR reagents such as internal controls etc.
  • Master Mix preparation in reaction with up to 6 components
Benefits
  • Highly flexible and user friendly system for a wide range of PCR types and protocols
  • Full reagent and labware flexibility, no supplier limitation
  • Reagent saving pipetting
  • Time saving due to reusable setups and optimized pipetting order
  • Traceability of all input parameters though the whole setup process
  • LIMS integration possible


Technical Data:

PCR setup STARlet

The PCR setup STARlet is available in 4 configurations, with 4 or 8 independent 1 ML pipetting channels and as manual load or autoload versions (with included barcode scanner).

 

PCR setup Software

The PCR setup software includes three different functions, two for PCR setup (quick/advanced assay design) and one for sample normalization. Each function can be purchased separately. The software can be used for implementation on the larger platforms STAR and STARplus and also for upgrading existing systems in the field.


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