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The NucleoTraP®CR extraction system is especially designed for direct purification of DNA fragments from PCR assays without gel electrophoresis. DNA is reversibly adsorbed to the matrix with the aid of chaotropic agents.

The silica-matrix based kit for PCR clean-up without columns

• Scalable batch binding procedure
• Quantitative removal of primer and primer-dimer molecules
• High recovery for DNA fragments > 120 bp

The NucleoTraP®CR matrix can selectively bind DNA fragments > 100 bp, while the primer and primer dimer molecules remain in solution. After two washing steps the DNA is eluted in TE buffer (10 mM Tris/HCl, 1 mM EDTA, pH 8.0) or another low-salt medium (e.g., water, pH 8.0–8.5).
The eluted DNA shows a very high purity. All impurities such as enzymes, nucleotides and the primer molecules are quantitatively removed. The eluted DNA can be used directly for all kinds of cloning experiments (ligation reactions, labelling reactions). DNA purified with NucleoTraP®CR gives excellent results in sequencing experiments using radioactive or fluorescent detection methods.

NucleoTraP®CR procedure

Technical Data:

Technology Silica-matrix technology
Format Silica bead suspension
Sample material < 400 µL PCR reaction mixture
Fragment size 100 bp–approx. 50 kbp
Typical recovery 70–80%
A260/A280 1.7–1.9
Elution volume 20–50 µL
Preparation time 45 min/6 preps
Binding capacity 0.6 µg/10 µL suspension

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