- Get Quote
The NucleoTraP®CR extraction system is especially designed for direct purification of DNA fragments from PCR assays without gel electrophoresis. DNA is reversibly adsorbed to the matrix with the aid of chaotropic agents.
The silica-matrix based kit for PCR clean-up without columns
|• Scalable batch binding procedure
• Quantitative removal of primer and primer-dimer molecules
• High recovery for DNA fragments > 120 bp
The eluted DNA shows a very high purity. All impurities such as enzymes, nucleotides and the primer molecules are quantitatively removed. The eluted DNA can be used directly for all kinds of cloning experiments (ligation reactions, labelling reactions). DNA purified with NucleoTraP®CR gives excellent results in sequencing experiments using radioactive or fluorescent detection methods.
|Format||Silica bead suspension|
|Sample material||< 400 µL PCR reaction mixture|
|Fragment size||100 bp–approx. 50 kbp|
|Elution volume||20–50 µL|
|Preparation time||45 min/6 preps|
|Binding capacity||0.6 µg/10 µL suspension|