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NucleoSpin® RNA

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Mini spin kits for the isolation of RNA of highest integrity

 

*Previously called NucleoSpin RNA II


 

  • rDNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
  • NucleoSpin® Filters (shredders) – efficient sample homogenisation and reduction of viscosity
  • Up to 70 μg ready-to-use RNA


Applications

  • RNA isolation from cultured cells, tissue (standard protocol)
  • RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
  • RNA from ≤ 100 μL biological fluids
  • RNA clean-up from reaction mixtures
  • RNA from samples stored in RNAlater®
  • RNA from saliva samples collected with Oragene®RNA (Genotek)
  • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

 

Technical Data:

Technology Silica-membrane technology
Format Mini spin columns
Sample material < 5 x 106 cultured cells,
< 109 bacterial cells,
< 108 yeast cells,
< 30 mg human / animal tissue
Fragment size > 200 nt
Typical yield 14 μg (106 HeLa cells), 70 μg (109 bacterial cells)
A260/A280 1.9–2.1
Typical RIN (RNA integrity number) > 9
Elution volume 40–120 µL
Preparation time 35 min/6 preps
Binding capacity 200 µg


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