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NucleoSEQ

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The NucleoSEQ® column the small dye terminators and other impurities, for example salts, nucleotides, primers, traces of organic solvents are retained in the pores while labeled DNA fragments are excluded and recovered in the flow-through with high yield. In order to achieve long-time storage at room temperature NucleoSEQ® columns are prefilled with dry gel filtration resin.


Pre-filled single spin columns for dye-terminator removal

• Efficient removal of dye terminators (e.g., BigDye™ terminators) without ethanol precipitation
• Convenient spin column format
• Long-term storage at room temperature

Characterization:

NucleoSEQ® columns are designed for fast, effective and cost efficient clean-up of sequencing reactions. The spin columns are prefilled with a dry size exclusion matrix which allows an efficient removal of dye terminators, for example BigDye™ terminators: the gel-filtration material consists of spheres with uniform pores and separates molecules according to molecular weight. After applying the seqencing reaction to the NucleoSEQ® column the small dye terminators and other impurities, for example salts, nucleotides, primers, traces of organic solvents are retained in the pores while labeled DNA fragments are excluded and recovered in the flow-through with high yield. In order to achieve long-time storage at room temperature NucleoSEQ® columns are prefilled with dry gel filtration resin.

 

NucleoSEQ®  principle

NucleoSEQ®  procedure

Application data

Sequencing profile of plasmid DNA (pGEM®-T Easy)
Plasmid DNA was purified using NucleoSpin® Plasmid. Sequencing reaction was performed with ABI PRISM®BigDye™ Terminator Cycle Sequencing kit, purified with NucleoSEQ®, and analyzed on an ABI 310 sequencer.

Click for downloading
the sequencing profile
as pdf file

 

 


 


Technical Data:

Ordering information

Product

Preps

Specification REF
NucleoSEQ®

10

pre-filled NucleoSEQ® Columns, Collection Tubes (2 mL)

50

as above

250

as above

 


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