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NucleoMag

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Magnetic-bead based PCR clean-up
• Closed system: reduced risk of cross-contamination
• Small elution volumes ≥ 25 μL
• Recovery does not depend on elution volume
• Easily adapted to automated use

Characterization:

With NucleoMag® 96 PCR a high recovery even for small fragments is achieved. Recovery depends on fragment length. On average, a recovery of 80–100% is observed.
• Closed system – reduced risk of cross-contamination
• Small elution volumes > 25 μL
• Recovery does not depend on elution volume
• Easily adapted to automated use
Technology Magnetic-bead technology
Format Highly reactive super paramagnetic beads
Processing Manual or automated
Sample material

< 50 µL PCR reaction mixture

Fragment size 150 bp–10 kbp
Typical recovery 80–95%
A260/A280 1.70–1.90
Elution volume 25–100 µL
Preparation time 30–45 min/96 preps
Binding capacity 0.3 µg/µL beads
Applications
• Manual or automated PCR clean-up
• 
Typical downstream application: sequencing 


Technical Data:

NucleoMag® 96 PCR provides a reliable, cost- and labor-efficient tool. PCR products are selectively bound to the NucleoMag® PCR beads while contaminants (e.g., primers, dNTPs, enzymes, salts) are washed away. Purified PCR products are eluted from the beads under low-salt conditions and are ready-to-use in downstream applications like sequencing or microarray spotting. As the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation) there is no risk of cross-contamination.

 

NucleoMag® principle

 

DNAcontaminants

NucleoMag® beads
NucleoMag® beads are added to the sample (e.g., lysed blood cells, PCR reactions) 

 

DNA is bound to the NucleoMag® beads. Beads are held in the well by the magnet while contaminants are washed away. 

 

 

DNA is eluted from the beads and recovered, while beads are held in the well by the magnet. DNA is ready-to-use in downstream applications

 

NucleoMag® 96 PCR procedure

 


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