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NucleoBond® Finalizer

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The NucleoBond® Finalizers are designed for quick concentration and desalination of plasmid and cosmid DNA eluates that are obtained by anion-exchange chromatographic DNA purification with NucleoBond® columns. The sample is precipitated with isopropanol and loaded onto a NucleoBond® Finalizer membrane by means of a syringe. After an ethanolic washing step and drying of the membrane the pure DNA can be eluted with low salt buffer for further use.


Plasmid DNA concentration and desalting

  • NucleoBond Finalizer,
  • FInalizer Plus,
  • Finalizer Large,
  • Finalizer Large Plus
Characterization:

Desalting tools for speeding up the time-consuming precipitation step after anion-exchange plasmid purification.
Eliminates centrifugation steps for precipitation – time saving from >1 h to only 5

min
• Two sizes available, to be combined with Midi and Maxi preparations
• Highly concentrated plasmid DNA
• No loss of DNA pellets or incomplete solubilization of hardly visible precipitates

 

 


 

The NucleoBond® Finalizer technology replaces the tedious and time-consuming centrifugation step which hitherto follows every isopropanol precipitation of diluted DNA samples. Furthermore problems like lost DNA pellets or incomplete solubilization of hardly visible precipitates can easily be circumvented. The pure concentrated and almost salt free DNA can be used directly for all common down-stream applications like transfection, in vitro transcription, automated or manual sequencing, cloning, hybridization, and PCR.

NucleoBond® Finalizer procedure

 


Technical Data:

NucleoBond® Finalizer

NucleoBond® Finalizer Large

Format Syringe filter
Sample material Plasmid and cosmid DNA eluates obtained by
anion-exchange chromatography
Vector size 2–50 kbp

2–50 kbp

Typical recovery 40–90%

40–90%

Typical concentration
(elution volume dependent)
0.1–3 µg/µL

0.1–3 µg/µL

Elution volume > 200 µ/L

> 400 µL

Preparation time 5 min

5 min

Residual chloride concentration < 0.3 µg/µL

< 0.3 µg/µL

Binding capacity 500 µg 2000 µg
To be combined with

NucleoBond® PC 100
NucleoBond® PC 500
NucleoBond® PC 500 EF
NucleoBond® Xtra Midi
NucleoBond® Xtra Midi EF

NucleoBond® Xtra Maxi
NucleoBond® Xtra Maxi EF
NucleoBond® PC 2000
NucleoBond® PC 2000 EF

Applications
• Quick concentration and desalination of plasmid and cosmid DNA eluates obtained by anion-exchange
chromatography
• Typical downstream applications: transfection, in-vitro transcription, automated or manual sequencing,
cloning, hybridization, and PCR   


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