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Microsep™ Advance Centrifugal Devices

  • Description
  • Specifications
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Precise, Quick Recovery of Microliter Volumes

  • Achieve 50X concentration and > 90% recovery in just minutes
  • Deadstop feature prevents samples from spinning to dryness
  • Versatile Omega™ membrane is available in a variety of MWCOs
  • Color-coded and laser etched for easy identification


Ultrafiltration

  • Concentrate dilute protein samples prior to electrophoresis
  • Exchange buffer and remove salt in samples
  • Isolate low molecular weight compounds from fermentation broths for natural product screening
  • Recover biomolecules from cell culture supernatants or lysates

Microfiltration

  • Clarify samples with gross particulate
  • Remove particulate from samples for HPLC analysis of drugs, amino acids, and antibodies
Characterization:

Materials of Construction

  • Filter Media: Omega (modified polyethersulfone) and Supor® (polyethersulfone) membranes
  • Sample Reservoir, Filtrate Receiver, and Cap: Polypropylene
  • Paddle: Polyethylene

Effective Filtration Area

  • 3.3 cm2

Dimensions

  • Diameter: 17 mm (0.7 in.)
  • Length: 12.0 cm (4.9 in.)

Capacities

  • Maximum Sample Volume: 5.0 mL
  • Final Concentrate Volume:
    65 μL (swinging bucket)
    80 μL (45° angle rotor)
    100 μL (34° angle rotor)
  • Filtrate Receiver Volume: 6.5 mL
  • Hold-Up Volume: 40 μL (membrane and paddle)

Operating Temperature Range

  • 0 – 40 °C (32 – 104 °F)

pH Range

  • 1 – 14

Maximum Centrifugal Force

  • 7,500 x g (ultrafiltration)
  • 14,000 x g (microfiltration)

Centrifuge

  • A fixed angle rotor or swinging bucket that accepts standard 17 x 100 mm tubes and is capable of 3,000 to 14,000 x g

Sanitization

  • Provided non-sterile. May be sanitized by filtering 70% ethanol through the device prior to use.


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