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Micro-24 MicroReactor System

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The Pall Micro-24 MicroReactor system is a compact solution for efficient cell line evaluation and process development. With options for a variety of culture conditions including aerobic & anaerobic microbial fermentation, mammalian & insect cell culture, the Micro-24 System offers the most flexible operating conditions available. The Micro-24 MicroReactor system runs up to 24 simultaneous bioreactor experiments with independent control and monitoring of each reactor’s gas supply, temperature and pH. It is designed for easy set-up and operation using single-use cassettes and closures.


Product Applications

Pall Micro-24 MicroReactor systems are in use worldwide to grow a wide range of cell types under highly diverse conditions. Our systems offer significant advantages over shake flask screening for fast growing organisms and high output processes In addition to the highly aerobic processes common to industrial fermentation, Pall MicroReactor systems are also being used to study environmentally interesting organisms under controlled anaerobic and micro-aerobic conditions. The Micro-24 MicroReactor system also excels in the execution of longer term mammalian and insect cell culture. Efficient mixing is delivered in a low shear environment providing high throughput capabilities for controlled process development. The superior mixing provided by the Micro-24 MicroReactor system allows researchers the flexibility to optimize processes for adherent cells on microcarriers as well as suspension cell culture.

Clone Selection

For most companies, clone production easily outpaces the available resources for data driven evaluation. The Pall Micro-24 MicroReactor technology offers the ability to screen hundreds to thousands of clones in controlled and monitored reactors. Thorough evaluation in a controlled reactor dramatically enhances the chances of selecting the best clones and minimizes the risks of unexpected performance during scale-up.

Media development and optimization

Rapidly screen a wide range of media and components in relevant conditions. Determine critical media components, trace nutrient fate using costly labeled substrates, and screen multiple microcarriers in parallel. Process control with minimal downtime ensures that studies are rapid and decisive.

Parametric optimization

From characterization of growth and specific productivity across a range of conditions to studying the timing of induction or temperature shifts on final productivity, Pall Micro-24 MicroReactors can provide critical process insights faster and earlier in process development. The parallel nature of the reaction vessels make the system ideal for DoE studies and multivariate analysis.

Microproduction

There are thousands of uses for reproducible production of milligram quantities of target proteins. Common examples include characterization, crystallography, proteomic studies, or the generation of affinity tags. The small format and capability of the Micro-24 MicroBioreactor system makes it uniquely suited to generate large numbers of small protein samples.

Typical Application Organisms

  • Escherichia coli
  • Pseudomonas fluorescens
  • Saccharomyces cerevisiae
  • Pichia pastoris
  • Bacillus subtilis
  • Shewanella oneidensis MR-1
  • Aspergillus nidulans
  • Trichoderma reesei
  • Aspergillus oryzae
  • Cell-free expression systems
  • Mammalian suspension cultures such as CHO
  • Human T-cells
  • Mouse and Human stem cell lines
  • Blood cells
  • Vero cells
  • Insect cell lines
Characterization:

Features

 

  • Single-use MicroReactor Cassettes:
    • 24 reactors per cassette
    • pH and DO sensors pre-calibrated
    • Irradiated and ready to use
    • Working volume is 3 to 7 mL
  • Independent control of each MicroReactor:
    • Dissolved oxygen
    • pH
    • Temperature
  • System-level control of agitation
  • Real-time monitoring and control

The Micro-24 MicroReactor Instrument

The Micro-24 MicroReactor features an integrated vacuum system to secure the single-use cassette during operation. Efficient mixing is achieved through an orbiting baseplate . Mixing conditions can be varied to support processes from shear sensitive stem cells to high density microbial. Each reactor in the single use cassette features the following sensing and control features:

  • Gas injection port for up to three control gases
  • Individual heater for temperature control
  • Individual thermocouple for temperature monitoring
  • pH optical sensor
  • DO optical sensor

 

 

24-Well Cassette Options and Details

Three cassette designs provide a variety of process conditions.

  • Standard [REG]
  • Baffled [BFL]
  • Central Vent [PRC]

Polycarbonate, irradiated by e-beam

Pall Micro-24 MicroReactor Microbial Cassette: Individual Reactor Detail

Schematic diagram illustrating the functioning of the Micro-24 MicroReactor microbial cassettes. Each of the three gas feeds is controlled individually for each reactor. In microbial applications, pH is controlled with either dilute ammonia or carbon dioxide. Oxygen control is achieved with either nitrogen or oxygen. Gases are sparged directly into the culture medium through the gas-permeable hydrophobic membrane at the bottom of each reactor well.

Pall Micro-24 MicroReactor Cell Culture Cassette: Individual Reactor Detail

Schematic diagram illustrating the functioning of the Micro-24 MicroReactor cell culture cassette. Up to 3 gas feeds can be individually controlled per reactor.  In this design, gases pass through the sterilizing membrane into the vent tube bypassing the media. This headspace gassing design allows control gases to diffuse into the culture medium during orbital agitation eliminating the need for antifoam.

Four Options for Well Closures

Type A: Single-use with neoprene check valve and sterile membrane barrier

Type D: Single-use with sterile membrane barrier

Type E: Autoclavable, re-usable, with stainless steel check valve, no sterile membrane barrier

Irradiated Tape Closure: Single-use, good for high gas exchange conditions, short experiment durations

Recommended cassette/closure combination for specific applications

Culture Type Bacteria Yeast
Cell Density All OD600 < 40 OD600 > 40 All OD600 < 30 OD600 > 30
O2Conditions Anaerobic Aerobic Anaerobic Aerobic
pH Conditions 3.5 – 5.5 / 6.0 – 8.0 3.5 – 5.5 / 6.0 – 8.0 3.5 – 5.5 / 6.0 – 8.0 3.5 – 5.5 / 6.0 – 8.0 3.5 – 5.5 / 6.0 – 8.0 3.5 – 5.5 / 6.0 – 8.0
Cassette PRCLOW / PRC PRCLOW / PRC REGLOW / REG PRCLOW / PRC PRCLOW / PRC REGLOW / REG
Cap TypeA/E TypeA/E TypeD TypeA/E TypeA/E TypeD
Gas1 CO2 (optional)
Gas2 NH3/N2 NH3/Air NH3/N2 NH3/Air
Gas3 N2 O2 N2 O2



Culture Type Filamentous Fungi Cell Culture
Cell Density All All
O2 Conditions Anaerobic Aerobic < 50% DO > 50% DO
pH Conditions 3.5 – 5.5 / 6.0 – 8.0 3.5 – 5.5 / 6.0 – 8.0 6.0 – 8.0
Cassette BFLLOW / BFL BFLLOW / BFL PRC
Cap TypeA/E TypeD TypeA/E TypeD
Gas1 CO2 (optional) 20-50% CO2/N2
Gas2 NH3/N2 NH3/Air 5-8% CO2/N2
Gas3 N2 O2 40 – 60% O2/N2

 

CONFIGURATION SCREEN

Rapid experimental set-up






Technical Data:

Micro-24™ MicroReactor System Specification

Dissolved Oxygen
Air/oxygen feed 0 – 20 sccm
DO setpoint range 0 – 100% air saturation
DO accuracy ± 5% at 0% air saturation, ± 10% at 100% air saturation

pH
Standard pH sensor setpoint range 6.0 – 8.0
Low pH sensor setpoint range 3.5 – 5.5
pH sensitivity ± 0.1 pH unit

Temperature (per Well)
T setpoint range 18 °C – 45 °C
T sensitivity ± 0.1 °C
Maximum achievable T gradient * 10 °C
*appropriate set-up required for gradient operation

System
Shaker 0-800 rpm programmable; 5mm orbital throw
Power requirements 110V , 5 A  service,
or 220 V, 2 A service
Air requirements Clean Dry Instrument Air >0.000472 cms (1.0 CFM), 4.5-7.5 Bar (65-110 psi),  Control Gases 1-2.1 Bar (15-30 psi), ¼ in.  or 6mm  fittings
Size 480 mm x 280 mm x 432 mm (w x h x d)
System Weight 22.5 Kilograms


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