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Macrosep® Advance Centrifugal Devices

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Quickly Concentrates Up To
20 mL of Biological Sample

  • Rapidly concentrates 20 mL sample volumes to 0.5 mL
  • Provides high recoveries, typically > 90%
  • Low protein-binding Omega™ membrane and polypropylene housing minimize losses due to non-specific binding
  • Versatile Omega membrane is available in a variety of MWCOs
  • Built-in deadstop prevents spinning to dryness
  • Color-coded for easy identification


Ultrafiltration

  • Concentrate and desalt proteins
  • Exchange buffer or remove salt of chromatography eluates and gradient fractions
  • Recover proteins or other molecules from cell culture supernatants

Microfiltration

  • Remove particulate from aqueous solutions and clinical samples
Characterization:

Macrosep Advance Centrifugal Devices: Reduced Spin Time


Protein solutions were processed in each of the Macrosep Advance devices. Average time (minutes) is plotted against mL of remaining product to be filtered using a swinging bucket rotor at 5,000 x g. Solutions are 3K: Protamine Sulfate, 0.1% in 1X PBS; 10K: Cytochrome C, 0.025% in 1X PBS; 30K: IgG, 0.1% in 1X PBS; and 100K: Apoferritin, 0.1% in 1X PBS.

Macrosep Advance Centrifugal Devices: Retention Efficiency


Macrosep Advance Centrifugal Devices: Retention Efficiency

Protein solutions were processed in each of the Macrosep Advance devices. Average percent retention using a swinging bucket rotor at 5,000 x g is displayed for each MWCO. Solutions were 3K: Protamine Sulfate, 0.1% 1X PBS; 10K: Cytochrome C, 0.025% in 1X PBS; 30K: IgG, 0.1% in 1X PBS; and 100K: Apoferritin, 0.1% in 1X PBS.


Technical Data:

Materials of Construction

  • Filter Media: Omega (modified polyethersulfone) and Supor® (polyethersulfone) membranes
  • Sample Reservoir, Filtrate Receiver, and Cap: Polypropylene
  • Paddle: Polyethylene

Effective Filtration Area

  • 7.2 cm2

Dimensions

  • Diameter: 29 mm (1.2 in.)
  • Length: 12.0 cm (4.7 in.)

Operating Temperature Range

  • 0 – 40 °C (32 – 104 °F)

Capacities

  • Maximum Sample Volume: 20 mL
  • Final Concentrate Volume:
    450 μL (swinging bucket)
    1.2 – 1.5 mL (45º angle rotor)
    1.5 mL (34º angle rotor)
  • Filtrate Receiver Volume: 22 mL
  • Hold-Up Volume: 80 μL (membrane and paddle)

pH Range

  • 1 – 14

Maximum Centrifugal Force

  • 5,000 x g (ultrafiltration)
  • 14,000 x g (microfiltration)

Centrifuge

  • Fits centrifuges that accept standard 50 mL conical end tubes

Sanitization

  • Provided non-sterile. May be sanitized by filtering 70% ethanol through thedevice prior to use.


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