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ImageXpress Micro 4 High-Content Imaging System

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The ImageXpress® Micro 4 High-Content Imaging System represents the culmination of four generations of imaging expertise. The latest agile design allows you to boost your research with this faster-than-ever system, while providing the option to upgrade to confocal in the future to align with your research needs.

Capture images of whole organism and cellular or intracellular events with the ImageXpress Micro 4 system configured to suit your specific biological needs.  We offer unlimited configurability with user exchangeable filter cubes, the widest range of objective lenses and environmental control, transmitted light, confocal imaging, and fluidics options.


 

 

 

 

 

 

Characterization:

APPLICATIONS

 3d-spheroid
 3D Imaging

  • Monitor cell health kinetics under environmental control for hours or days
  • Reconstruct 3D spheroids with rapid z-stack imaging
  • Study spheroids with readily available plates and straightforward imaging workflow
  • Acquire from a variety of multi-well plate formats up to 1536 including unusual formats developed for 3D assays including round bottom and trans-well plates

 thick samples
 Thick Samples

  • Image neurons or stem cells grown on 3D matrices that can make focusing on the cells of interest a challenge
  • Capture difficult-to-image thick tissue sections (at least 20 µm thick) that produce high-background fluorescence when stained
  • Analyze 3D samples by collecting a stack of crisp images in the Z-plane in as small as 100 nm increments
whole organism
Whole Organism Imaging

  • Image an entire well
  • Keep organisms in focus for the duration of experiment
  • Perform sophisticated analysis of images and create time-lapse videos

Live cells

Above, see a 16-hour time-course movie of HeLa cells expressing Cell Cycle Chromobody (R). Notice that the cells are undergoing normal cell division while being imaged in confocal mode. In G1, the cells have a homogeneous fluorescence signal. During S phase, signal accumulates in the nucleus with formation of foci. In G2, the morphology returns to homogenous and the cell divides.

 Live Cells

  • Monitor cell growth, death, differentiation, and migration; viral or bacterial invasion, cancer metastasis, chemotaxis, drug toxicity, or translocation
  • Observe rapid kinetic cellular responses over a span of several days using fluorescent or unlabeled cells kept in an environmental control chamber, together with a brightfield microscope, and on-board fluid addition capabilities

Screening

Unstimulated transfluor-expressing cells wtih cytosolic distribution of beta arrestin GFP. Nuclei stained in blue, mitochondria in red.

Screening2

Stimulated transfluor-expressing cells with beta-arrestin GFP that has redistributed to vesicles. Nuclei stained in blue, Mitochondria in red.

Screening

  • Determine the mechanisms of action for the research of cancer, neurological disorders, viral infection, and other diseases with compound or RNAi screening
  • Quantitate multiplexed cellular responses, such as apoptosis, cell cycle, micronuclei, neurite outgrowth, angiogenesis, intracellular translocation, endocytosis, and cell signaling
  • Detect one to four fluorescent markers along with the ability to resolve their colocalization

RNA_interference1

Images of control cells showing DNA damage detected by H2AX phosphorylation. The positive control (bottom) clearly shows a higher percentage of cells with DNA damage (green) compared to total cells (red) in the well.

RNA_interference2

At higher magnification assays DNA foci indicating damage as marketed by H2AX phosporylation (green) and 53BP1 (red) are more easily resolved with confocal imaging.

RNA Interference

  • Interrogate large-scale RNAi libraries
  • Measure critical events including apoptosis, cell cycle, micronuclei, neurite outgrowth, angiogenesis, intracellular translocation, endocytosis, cell signaling, and multi-wavelength cell scoring.
  • Automatically identify, segment, and analyze biological phenotypes using validated software modules

StemCells

Differentiated neurons imaged in a 96-well plate at 10x magnification with three different fluorescent markers

 Stem Cell Research

  • Measure stem cell growth and differentiation in layers or colonies with the system’s widefield focusing capabilities, brightfield imaging option, and environmental control chamber for extended period of time
  • Accurately track cell processes, colony size or number across multiple fields of view with unique stitching algorithm and precise plate stage movements
  • Gain insight into the complex molecular and genetic signaling events that underlie cell proliferation and differentiation
  • Accurately measure nuronal process across multiple fields of view using unique stitching algorithm with precise stage movements
  • Analyze 3D samples by capturing an image stack in the Z-plane within each field of view and collapse it into a best-focus image or view the sample in 3D
Homogeneous Binding Assay

  • Multiplex different cells in the same plate well
  • Run assays with adherent cells, suspension cells, or beads
  • Detect fluorescent labels at any wavelength
  • Greater than 5 fold improved sensitivity in antibody binding assays in confocal over widefield

 


Technical Data:

Camera

 

  • 4.66 megapixel scientific CMOS

Stages

 

  • Linear encoded voice coil driven X,Y, and Z stages have < 100 nm resolution

Objectives

 

  • Variety of Nikon 1x- 100x air and oil immersion lenses are supported.

Standard Filter Options

 

Fluorophore
Part Number
Ex (nm) Dichroic (nm) EM (nm)
DAPI, Alexa Fluor 350, AMCA, BFP, Hoechst, LysoSensor Blue, Marina Blue, Pacific Blue, and sgBFP
1-6300-0442
377/50
327-404 (R)
415-950 (T)
447/60
CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1
1-6300-0447
438/24
426-450 (R)
467-950 (T)
483/32
GFP, Cy2™, CyQuant, DiO, EGFP, sgGFP™, wtGFP (non-UV excitation), YOYO-1, YO-PRO-1
1-6300-0450
472/30
350-488 (R)
502-950 (T)
520/35
FITC, FAM, Alexa Fluor 488, BODIPY, Calcein, Fluo-4, MitoTracker Green, Oregon Green, Rhodamine 110, rsGFP (red-shifted GFP, S65T)
1-6300-0443
482/35
350-500 (R)
513-950 (T)
536/40
YFP, Calcium Green-1, Eosin, Fluo-3, Rhodamine 123
1-6300-0448
500/24
350-512 (R)
528-950 (T)
542/27
TRITC, Alexa Fluor 546, 5-TAMRA, Alexa Fluor 532, SpectrumOrange™, BODIPY TMR, DiI, POPO-3, PO-PRO-3, Rhodamine
1-6300-0444
543/22
350-555 (R)
569-950 (T)
593/40
Cy3™, DsRed, Alexa Fluor 555, Calcium Orange, LysoTracker Yellow, MitoTracker Orange, Phycoerythrin (PE), Rhodamine Phallicidine, and TAMRA  
1-6300-0445
531/40
350-555 (R)
569-950 (T)
593/40
Texas Red®, mCherry, 5-ROX, Alexa Fluor 568 & 594, Calcium Crimson, Cy3.5™, HcRed, Propidium Iodide, and MitoTracker Red  
1-6300-0449
562/40
350-585 (R)
601-950 (T)
624/40
Cy5™, SpectrumFRed™, Alexa Fluor 647 & 660, APC (Allophycocyanin), BODIPY 650/665, DiD, SYTO Red, DRAQ5, TOTO-3, and TO-PRO-3  
1-6300-0446
628/40
350-651 (R)
669-950(T)
692/40

Additional filters as well as custom configurations are available upon request.
Filter specifications are current as of 9/1/2015.

Space And Weight Specifications

 

ImageXpress Micro 4 system configuration
W x D x H
Weight
ImageXpress Micro Base Unit System
21 x 34 x 19 (Inches)
53 x 86 x 48 (Centimeters)
215 (Lbs)
95 (Kg)
Transmitted Light and Live Cell (Environmental Control)
21 x 34 x 32 (Inches)
53 x 86 x 81 (Centimeters)
220 (Lbs)

100 (Kg)
Live Cell (Environmental Control) and Fluidics
21 x 34 x 42 (Inches)
53 x 86 x 107 (Centimeters)
295 (Lbs)

134 (Kg)
Transmitted Light
21 x 34 x 32 (Inches)
53 x 86 x 81 (Centimeters)
220 (Lbs)

100 (Kg)
Live Cell (Environmental Control)
21 x 34 x 19 (Inches)
53 x 86 x 48 (Centimeters)
210 (Lbs)

95 (Kg)


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