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Retention system for perfusion, concentration and washing

The Applikon BioSep system is a unique, cell retention device for high-density perfusion processes. Using high frequency resonant ultrasonic waves to separate cells instead of a physical mesh or membrane, it offers all the benefits of traditional devices but without their inherent problems and limitations.

The BioSep, based on the technology of acoustic resonance, is a non fouling / non clogging retention system. It can be applied in continuous mode which makes the BioSep the most favourable instrument for perfusion cultures.

Other applications for the BioSep:

  • cell concentration
  • cell washing

Features, Advantages and Benefits:

  • Proven under cGMP conditions
  • Cost effective
  • Easy to install and to operate
  • Applicable on any type and brand of bioreactor
  • long term cultivation possible (>60 days)
  • no fouling or blocking
  • no damage to the cells
  • increased cell viability
  • high separation efficiency


Technical Data:

Acoustic perfusion process

An acoustic perfusion process using the BioSep typically involves continuous addition of fresh medium to the bioreactor, while cells are filtered from the harvest stream by the resonator chamber and returned to the bioreactor. The BioSep can be directly mounted onto the bioreactor head plate. One standard mode of operation employs, for example, a harvest pump at the exit port of the resonator chamber, and a recirculation pump for the return of separated cells that settled from the acoustic energy field within the resonator chamber. Alternatively the BioSep system can also be set up to allow for semicontinuous operation or alternative strategies of cell recirculation.

Acoustic perfusion is generally applicable for suspended mammalian and animal cell culture, but can also be adopted for anchorage dependent cell lines, or the perfused culture of plant cells. The BioSep separation principle is purely based on gentle, acoustically induced loose aggregation followed by sedimentation. In contrast to other cell separation techniques, the acoustic energy mesh created within the BioSep constitutes “virtual”, thus superior-, non-contact, non-fouling, non-moving filtration means allowing for up to thousands of hours of continuous operation. As a result, greatly increased steady state cell density, productivity, and product quality is obtained.

The Biosep comes in different versions ranging from a capacity of 1L upto 1000L/day, divided over five different models. BioSep is using SonoSep Technology.

All systems can be used in re-circulation mode and in single pass mode.

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