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Agaroses

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Agarose is an essential tool in nucleic acid and protein separation in Genetic Engineering, Cell Culture and Microbiology.

Agarose is a natural product that forms an inert matrix used in Electrophoresis, Chromatography and other Molecular Biology and Biochemistry techniques. Likewise, it is neutral and easily derivatisable, so it is easy to bind to its structure proteins like enzymes, antigens or antibodies. Toxicity absence makes working with agarose very convenient.

 

 

 

 

Characterization:

Agarose is neutral and free from toxic material so it can be handled freely. In addition to its uses in gels,agarose can be used to form support structures such as beads, to which proteins, such as enzymes and antibodies, as well as other products, including dyes and antigens, can be fixed for separations. Agarose is an indispensable tool for Molecular Biology, Biochemistry, Cell Structure and Microbiology.


The structure of the polysaccharide is a galactan, formed by linking agarobioses 1-3, 1-4, as shown in the illustration. This chemical structure gives agarose the capacity to form gels that are very resistant even at low concentrations.

  • Dry powder, non hygroscopic
  • Electrically neutral
  • Biologically inert
  • Can be stored for several years, if stored properly
  • Free of toxicity (non neurotoxic as acrylamide)
  • Can be autoclaved for sterile applications
  • Easily derivatizable
  • No free-radicals/additives needed for gelification
  • Macroporous matrix – allows molecular diffusion
  • Easy preparation
  • Thermoreversible, gels formed by hydrogen bonds
  • Resistant, even at low concentrations
  • Thermal stability
  • Low absorption of staining agents-low background
  • Pore size changes only by changing concentration
  • Gels can be dried for recordkeeping

Agarose applications

  • Inmunodiffusion: In this technique, macromolecules migrate and are precipitated in the gel by molecular diffusion.
  • Electrophoresis: Agarose is suitable for the widest range of electrophoresis procedures as well as in immunoelectrophoresis and electrofocusing. Driven by electrostatic fields, the macromolecules migrate through the macroreticular structure.
  • Gel Chromatography, Affinity Chromatography and Ion Exchange Chromatography: In these applications, the movement of macromolecules is caused by the displacement of solvent along the gel formed in microspheres.

Selection guide

   STANDARD MELTING    LOW MELTING
DNA/RNA ≥ 1000 bp D1 LE (regular applications and blotting)
D1 LE GQT (preparative gels)
D1 ME (regular applications)
D2 (regular applications)
D5 (high mobility)
FP DNA (DNA typing)
LM (regular applications and blotting)
LM GQT (preparative gels)
DNA/RNA ≤ 1000 bp MS6 Metagel 
MS8 (fine resolution)
MS12 (regular applications and blotting)
LM Sieve (preparative)
MS4 (fine resolution)
NOVAGEL GQT (fine resolution)


Technical Data:

 


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