Open/Close Menu

Acrodisc® Units with Mustang® Q and S Membranes

  • Description
  • Specifications
  • Downloads
  • Get Quote

Disposable Chromatography Units with High Binding Capacities and Fast Flow Rates

  • Fast flow rates. Recommended flow rates of 1 to 4 mL/minute do not affect recovery rates.
  • Mustang Q membrane is a strong anion exchanger that effectively binds plasmid DNA, negatively-charged proteins, and viral particles.
  • Mustang S membrane is a strong cation exchanger that effectively binds positively-charged proteins and viral particles.
  • Disposable 25 mm units are designed for single laboratory use only.
  • Direct scale up to capsules with Mustang Q and S membranes for larger-volume applications.


Mustang Q Membrane

  • Provides contaminant removal such as DNA viral particle, host cell proteins, or endotoxin
  • Ideal for isolation via capture and release of plasmid DNA, virus, or target protein from a complex mixture
  • Offers protein polishing for negativelycharged proteins
  • Purifies virus and oligonucleotides

Mustang S Membrane

  • Purifies and concentrates positively-charged proteins and viral particles
  • Ideal for evaluation, process development, and validation
Characterization:

Acrodisc Unit with Mustang Q Membrane:
Resolution with BSA and Goat lgG


Acrodisc Units with Mustang Q Membranes

The conditions used to generate data for the resolution graph above include buffer: 25mM Tris pH 8.0; salt: 1M NaCl in 25mM Tris pH 8.0; gradient: 0 to 0.5M NaCl in 50 column volume (CV); flow rate: 2.3 mL/min (13 cv/min); sample loading: 4% of total binding capacity.

Acrodisc Unit with Mustang Q Membrane:
Dynamic Binding with BSA


Acrodisc Units with Mustang Q Membranes Dynamic Binding

A solution of 0.524 mg/mL BSA was pumped through the Acrodisc unit at 2.3 mL/min. Breakthrough occurred at 8.1 minutes and was calculated as 54 mg/mL using:
flow rate (2.3 mL/min) X initial protein BSA concentration (0.524 mg/mL) X time (8.1 min)
membrane bed volume of Mustang Q membrane in 25 mm Acrodisc unit (0.18 mL)

Acrodisc Unit with Mustang S Membrane:
Resolution with Cytochrome C and Lysozyme


Acrodisc Unit with Mustang S Membrane: Resolution with Cytochrome C and Lysozyme

The conditions used to generate data for the graph above include buffer: 10mM MES pH 5.5; salt: 1M NaCl in 10mM MES pH 5.5; gradient: 0 to 1M NaCl in 50 CV; flow rate: 2.3 mL/min (13 cv/min).

Acrodisc Unit with Mustang S Membrane:
Dynamic Binding with Lysozyme


 

A solution of 0.512 mg/mL Lysozyme was pumped through the Acrodisc unit at 2.3 mL/min. Breakthrough occurred at 8.0 minutes and was calculated as 52 mg/mL using:
flow rate (2.3 mL/min) X initial protein Lysozyme concentration (0.512 mg/mL) X time (8.0 min)
membrane bed volume of Mustang S membrane in 25 mm Acrodisc unit (0.18 mL)


Technical Data:

Materials of Construction

  • Filter Media: Mustang Q and Mustang S membranes
  • Housing: Polypropylene

Pore Size

  • 0.8 μm

Membrane Bed Volume

  • 0.18 mL

Inlet/Outlet Connections

  • Female luer lock inlet, male slip luer outlet

Maximum Operating Pressure

  • 5.5 bar (550 kPa, 80 psi) at ambient temperature 21 – 24 ºC (70 – 75 ºF)

Biological Safety

  • Passes United States Pharmacopeia (USP) Biological Reactivity Test, In Vivo <88>. Mustang Q membrane testing performed at 72 ºC (161 ºF) and Mustang S membrane at 50 ºC (122 ºF) after preconditioning.

Typical Operating Temperature

  • 21 – 24 ºC (70 – 75 ºF)

Recommended Flow Rate

  • 1 – 4 mL/min; flow rate will vary with type of solutions, concentration of proteins, and other components.

Typical Mean Dynamic Binding Capacity1

  • Mustang Q Membrane
    • DNA: 3.6 mg/Acrodisc unit or 30 mg/mL membrane volume (mv)
    • BSA: 10 mg/Acrodisc unit or 70 mg/mL membrane volume (mv)
  • Mustang S Membrane
    • Lysozyme: 8 mg per Acrodisc unit or 47 mg/mL membrane volume (mv)
    • Human IgG: 11 mg per Acrodisc unit or 60 mg/mL membrane volume (mv)

1Note on optimization: The yield is contingent on type of DNA, size and copy number of plasmid, concentration of protein, ionic strength, and pH of buffer.


You have nothing in your basket. Please click on the ‘Add To Cart’ button to request a quotation!